Single-Cell Oil Production by Engineered Ashbya gossypii from Non-Detoxified Lignocellulosic Biomass Hydrolysate

In this work, microbial lipid production from non-detoxified Eucalyptus bark hydrolysate (EBH) with oleaginous xylose-utilizing Ashbya gossypii strains was explored. The best producing strain a set of engineered identified in synthetic media mimicking the composition EBH (SM), profile characterized, and yeast extract corn steep liquor (CSL) were pinpointed as supplements enabling good balance between accumulation, biomass production, autolysis by A. gossypii. potential A877 to produce lipids further validated optimized minimally processed inhibitor-containing high sugar concentration, scaled up 2 L bioreactor. Lipid supplemented CSL reached titer 1.42 g/L, paving way for sustainable single-cell oil within concept circular economy placing an alternative by-product biorefineries.